Article
The DNA isolation method has effect on allele drop-out and on the results of fluorescent PCR and DNA fragment analysis.
Clinica chimica acta; international journal of clinical chemistry - 1 Oct 2005
Nagy Bálint, Bán Zoltán, Papp Zoltán
Abstract excerpt
BACKGROUND: The quality and the quantity of isolated DNA have an effect on PCR amplifications. METHODS: The authors studied three DNA isolation protocols (resin binding method using fresh and frozen amniotic fluid samples, and silica adsorption method using fresh samples) on the quantity and on the quality of the isolated DNA. Amniotic fluid samples were obtained from 20 pregnant women. The isolated DNA...
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