Article
Rapid determination of trisomy 21 from amniotic fluid cells using single-nucleotide polymorphic loci.
Prenatal diagnosis - 1 Dec 2005
Nagy Bálint, Bán Zoltán, Lázár Levente, Nagy Richárd Gyula, Papp Csaba, Tóth-Pál Erno, Papp Zoltán
Abstract excerpt
OBJECTIVES: Rapid detection of trisomy 21 is an important goal for prenatal genetic centers. Fluorescent-PCR and DNA fragment analysis was developed a decade ago and thousands of samples were analyzed in routine practice using this method. Quantitative real-time PCR with melting curve analysis using SNP markers for trisomy 21 detection was described recently. We studied the reliability of this method on a cohort...
Topics
- Alleles
- Amniotic Fluid
- DNA
- Down Syndrome
- Genetic Testing
- Humans
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide
- Prenatal Diagnosis
- Reproducibility of Results
- Time Factors