Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Gene replacement in Haloarcula marismortui: construction of a strain with two of its three chromosomal rRNA operons deleted.

Extremophiles : life under extreme conditions - 1 Dec 2005

Tu Daqi, Blaha Gregor, Moore Peter B, Steitz Thomas A

Abstract excerpt

Site-directed mutagenesis were done in Haloarcula marismortui using the strategy that Khorana and coworkers devised for deleting the bacteriorhodopsin gene from Halobacterium halobium [Krebs et al. Proc Natl Acad Sci USA 90:1987-1991 (1993)]. Strains have been prepared from H. marsimortui, which normally has three rRNA operons, that are missing either its rrnB operon or both its rrnB and rrnC operons. In rich...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Electrons
Share to Genetic Techniques

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Models, Chemical

Share to Ribosomal Proteins

Share to RNA, Ribosomal

Share to RNA, Ribosomal, 23S

Share to rRNA Operon

Share to Sucrose

Source

Identifiers

PMID: 15970993
DOI: 10.1007/s00792-005-0459-y

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Gene replacement in Haloarcula marismortui: construction of a strain with two of its three chromosomal rRNA operons deleted.PMID 15970993Read ↗

Sequence heterogeneity between the two genes encoding 16S rRNA from the halophilic archaebacterium Haloarcula marismortui.Semantic proximity Read ↗

Intragenomic 16S rDNA divergence in Haloarcula marismortui is an adaptation to different temperatures.Semantic proximity Read ↗

A genetic strategy for probing the functional diversity of magnetosome formation.Semantic proximity Read ↗

Identification and elimination of genomic regions irrelevant for magnetosome biosynthesis by large-scale deletion in Magnetospirillum gryphiswaldense.Semantic proximity Read ↗

Homologous gene knockout in the archaeon Halobacterium salinarum with ura3 as a counterselectable marker.Semantic proximity Read ↗

Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.Semantic proximity Read ↗

Genetic screen for regulatory mutations in Methanococcus maripaludis and its use in identification of induction-deficient mutants of the euryarchaeal repressor NrpR.Semantic proximity Read ↗

Mutations outside the anisomycin-binding site can make ribosomes drug-resistant.Semantic proximity Read ↗

A cyanobacterial strain with all chromosomal rRNA operons inactivated: a single nucleotide mutation of 23S rRNA confers temperature-sensitive phenotypes.Semantic proximity Read ↗

Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants.Semantic proximity Read ↗

A halorhodopsin-overproducing mutant isolated from an extremely haloalkaliphilic archaeon Natronomonas pharaonis.Semantic proximity Read ↗

Phenotypic evidence that the function of the [Fe]-hydrogenase Hmd in Methanococcus maripaludis requires seven hcg (hmd co-occurring genes) but not hmdII.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.