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Analysis of the large bowel microbiota of colitic mice using PCR/DGGE

16 Jun 2005

Abstract excerpt

AIM: To test combined polymerase chain reaction amplification of 16S rRNA gene sequences and denaturing gradient gel electrophoresis (PCR/DGGE) as an analytical method to investigate the composition of the large bowel microbiota of mice during the development of colitis. METHODS AND RESULTS: The colonic microbiota of formerly germfree interleukin 10 (IL-10)-deficient mice that had been exposed to the faecal...

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PMID: 15960751
DOI: 10.1111/j.1472-765x.2005.01720.x

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