Article
Isolation and sequencing of CA/GT repeat microsatellites from chromosomal libraries without subcloning.
Analytical biochemistry - 1 Jan 1992
Taylor G R, Haward S, Noble J S, Murday V
Abstract excerpt
A method for the isolation of (CA)n microsatellites from chromosome-specific genomic libraries is described. Clones were first screened using a polynucleotide CA/GT probe. Those shown to contain CA repeats were plaque purified and either subcloned or the insert amplified directly using vector pri...
Topics
- Alleles
- Animals
- Base Sequence
- Chromosomes, Human, Pair 21
- Cloning, Molecular
- DNA, Satellite
- Genetic Vectors
- Humans
- Hybrid Cells
- Mice
- Molecular Sequence Data
- Oligonucleotides
- Polymerase Chain Reaction
- Repetitive Sequences, Nucleic Acid
- Temperature