Article
Isolation and sequencing of CA/GT repeat microsatellites from chromosomal libraries without subcloning.
Analytical biochemistry - 1 Jan 1992
Taylor G R, Haward S, Noble J S, Murday V
Abstract excerpt
A method for the isolation of (CA)n microsatellites from chromosome-specific genomic libraries is described. Clones were first screened using a polynucleotide CA/GT probe. Those shown to contain CA repeats were plaque purified and either subcloned or the insert amplified directly using vector primers. Polymerase chain reaction products were then used to directly sequence the regions flanking CA repeats by using...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.