Article
Development of a real-time, TaqMan reverse transcription-PCR assay for detection and differentiation of lyssavirus genotypes 1, 5, and 6.
Journal of clinical microbiology - 1 Jun 2005
Wakeley P R, Johnson N, McElhinney L M, Marston D, Sawyer J, Fooks A R
Abstract excerpt
Several reverse transcription-PCR (RT-PCR) methods have been reported for the detection of rabies and rabies-related viruses. These methods invariably involve multiple transfers of nucleic acids between different tubes, with the risk of contamination leading to the production of false-positive results. Here we describe a single, closed-tube, nonnested RT-PCR with TaqMan technology that distinguishes between...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
