Article
Evaluation of Two Real-Time, TaqMan Reverse Transcription-PCR Assays for Detection of Rabies Virus in Circulating Variants from Argentina: Influence of Sequence Variation.
Viruses - 25 Dec 2020
Caraballo Diego A, Lombardo María A, Becker Paula, Sabio María S, Lema Cristina, Martínez Leila M, Beltrán Fernando J, Li Yu, Cisterna Daniel M
Abstract excerpt
In rabies diagnosis, it is essential to count on a rapid test to give a quick response. The combined sensitivity and robustness of the TaqMan RT-PCR assays (qRT-PCR) have made these methods a valuable alternative for rabies virus (RABV) detection. We conducted a study to compare the applicability of two widely used qRT-PCR assays targeting the nucleoprotein gene (LysGT1 assay) and leader sequences (LN34 qRT-PCR...
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