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Article

Rapid detection of rabies and rabies-related viruses by RT-PCR and enzyme-linked immunosorbent assay.

Journal of virological methods - 1 Dec 1997

Whitby J E, Heaton P R, Whitby H E, O'Sullivan E, Johnstone P

Abstract excerpt

A rapid detection method for the six established genotypes of rabies and rabies-related viral RNA using RT-PCR-ELISA is described. The detection of digoxigenin-labelled amplified products is performed by solution hybridization to two specific, biotin-labelled, capture probes, which are complementary to the inner region of the amplification products. The capture probe and amplified product hybrid are then...

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PMID: 9504752
DOI: 10.1016/s0166-0934(97)00143-2

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