Article
Detection of single nucleotide polymorphism in the FUT2 gene by temperature gradient gel electrophoresis.
Legal medicine (Tokyo, Japan) - 1 Jul 2005
Mitani Tomoaki, Akane Atsushi
Abstract excerpt
A simple and rapid analysis system for single nucleotide polymorphisms (SNPs) was investigated for the FUT2 gene using the temperature gradient gel electrophoresis (TGGE) method. The 426-bp or 259-bp FUT2 fragments were amplified from heterozygous samples using primers, and the heteroduplex and homoduplex bands were detected by TGGE. The FUT2 fragments amplified from homozygous samples were denatured and...
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