Article
Electron transfer chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium.
The FEBS journal - 1 Jun 2005
Igarashi Kiyohiko, Yoshida Makoto, Matsumura Hirotoshi, Nakamura Nobuhumi, Ohno Hiroyuki, Samejima Masahiro, Nishino Takeshi
Abstract excerpt
Cellobiose dehydrogenase (CDH) is an extracellular flavocytochrome containing flavin and b-type heme, and plays a key role in cellulose degradation by filamentous fungi. To investigate intermolecular electron transfer from CDH to cytochrome c, Phe166, which is located in the cytochrome domain and approaches one of propionates of heme, was mutated to Tyr, and the thermodynamic and kinetic properties of the mutant...
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