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Use of Heterologous Complementary DNA Array Screening to Analyze Bovine Oocyte Transcriptome and Its Evolution During In Vitro Maturation1

1 Jan 2003

Abstract excerpt

We have analyzed gene expression in bovine oocytes before and after in vitro maturation (IVM) using heterologous hybridization onto cDNA array. Total RNA was purified from pools of over 200 oocytes either immediately after aspiration from follicles at the surface of slaughterhouse cow ovaries or following in vitro maturation. Radiolabeled cDNA probes were generated by reverse-transcription followed by linear PCR...

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PMID: 12493721
DOI: 10.1095/biolreprod.102.007872

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