Article
Detection of a molecular defect in 40 of 44 patients with haemophilia B by PCR and denaturing gradient gel electrophoresis.
British journal of haematology - 1 Aug 1993
Tartary M, Vidaud D, Piao Y, Costa J M, Bahnak B R, Fressinaud E, Congard B, Laurian Y, Meyer D, Lavergne J M
Abstract excerpt
Oligonucleotides were computer designed to amplify by the polymerase chain reaction (PCR) the coding region, splice junctions, 112 bp of the 5' flanking region and 279 bp surrounding the polyadenylation site of the factor IX gene for analysis by denaturing gradient gel electrophoresis (DGGE). For...
Topics
- Amino Acids
- Base Sequence
- Blotting, Southern
- Child
- DNA
- Electrophoresis, Polyacrylamide Gel
- Factor IX
- Hemophilia B
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction