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Article

Detection of a molecular defect in 40 of 44 patients with haemophilia B by PCR and denaturing gradient gel electrophoresis.

British journal of haematology - 1 Aug 1993

Tartary M, Vidaud D, Piao Y, Costa J M, Bahnak B R, Fressinaud E, Congard B, Laurian Y, Meyer D, Lavergne J M

Abstract excerpt

Oligonucleotides were computer designed to amplify by the polymerase chain reaction (PCR) the coding region, splice junctions, 112 bp of the 5' flanking region and 279 bp surrounding the polyadenylation site of the factor IX gene for analysis by denaturing gradient gel electrophoresis (DGGE). Forty-four unselected haemophilia B patients were studied of whom 24 had severe haemophilia and 20 had a mild to moderate...

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PMID: 8217825
DOI: 10.1111/j.1365-2141.1993.tb03143.x

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Detection of a molecular defect in 40 of 44 patients with haemophilia B by PCR and denaturing gradient gel electrophoresis.PMID 8217825Read ↗

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