Article
Effects of disrupting the 21 kDa subunit of complex I from Neurospora crassa.
The Biochemical journal - 15 Sept 1999
Ferreirinha F, Duarte M, Melo A M, Videira A
Abstract excerpt
We have cloned and inactivated in vivo, by repeat-induced point mutations, the nuclear gene encoding a 21 kDa subunit of complex I from Neurospora crassa. Mitochondria from the nuo21 mutant lack this specific protein but retain other subunits of complex I in approximately normal amounts. In addition, this mutant is able to assemble an almost intact enzyme. The electron transfer activities from NADH to artificial...
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