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Article

Genotyping the hemophilia inversion hotspot by use of inverse PCR.

Clinical chemistry - 1 Jul 2005

Rossetti Liliana Carmen, Radic Claudia Pamela, Larripa Irene Beatriz, De Brasi Carlos Daniel

Abstract excerpt

BACKGROUND: Factor VIII intron 22 inversions (Inv22) cause 40%-45% of severe cases of hemophilia A in all human populations. Currently, Inv22 can be analyzed either by Southern blotting or by rapid long-distance-PCR-based approaches. We describe an alternative method using inverse-PCR (I-PCR). METHODS: I-PCR involved 3 steps: (a) BclI restriction; (b) self-ligation of restriction fragments, providing BclI rings;...

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Source

Identifiers

PMID: 15860568
DOI: 10.1373/clinchem.2004.046490

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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