Article
[Construction of fused osmoregulation proBA gene from a salt-tolerant mutant of Bacillus subtilis and its influence on the osmotolerance of Escherichia coli].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Feb 2005
Liu Rui-jie, Chen Ting, Cao Jun-wei
Abstract excerpt
The osmoregulation proB and proA genes from Bacillus subtilis 93151 are overlapping genes, which encode two proteins ProB and ProA. A restriction enzyme site was inserted in the overlapping region of proB and proA genes from a salt-tolerant mutant of B. subtilis 93151, and a fusion gene was constructed by cloning proB and proA genes respectively. SDS-PAGE analysis showed that a novel protein with molecular mass...
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