Article
An in vitro method for analysis of chondrogenesis in limb mesenchyme from individual transgenic (hdf) embryos.
Methods in cell science : an official journal of the Society for In Vitro Biology - 1 Jan 2003
Gillotte Danielle M, Fox Patricia L, Mjaatvedt Corey H, Hoffman Stanley, Capehart Anthony A
Abstract excerpt
The present study describes a simple, rapid protocol for culture for limb tissue from individual 10.5-day post coitum mouse embryos that supports cartilage differentiation over a six-day period. This technique differs from other commonly used methods utilizing pooled limb tissue in that: 1) forelimbs from individual embryos were used as donor tissue; 2) limb tissue was dissociated by very gentle enzymatic digest...
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