Article
Clinical implications of promoter hypermethylation in RASSF1A and MGMT in retinoblastoma.
Neoplasia (New York, N.Y.) - 1 Mar 2005
Choy Kwong Wai, Lee Tom C, Cheung Kin Fai, Fan Dorothy S P, Lo Kwok Wai, Beaverson Katherine L, Abramson David H, Lam Dennis S C, Yu Christopher B O, Pang Chi Pui
Abstract excerpt
We investigated the epigenetic silencing and genetic changes of the RAS-associated domain family 1A (RASSF1A) gene and the O6-methylguanine-DNA methyltransferase (MGMT) gene in retinoblastoma. We extracted DNA from microdissected tumor and normal retina tissues of the same patient in 68 retinoblastoma cases. Promoter methylation in RASSF1A and MGMT was analyzed by methylation-specific PCR, RASSF1A sequence...
Topics
- Cell Cycle
- Cell Line, Tumor
- Cell Proliferation
- CpG Islands
- DNA
- DNA Methylation
- DNA Mutational Analysis
- Disease Progression
- Exons
- Flow Cytometry
- Humans