Article
Epitope mapping of human respiratory syncytial virus 22K transcription antitermination factor: role of N-terminal sequences in protein folding.
The Journal of general virology - 1 Apr 2005
García-Barreno Blanca, Steel John, Payá Monica, Martínez-Sobrido Luis, Delgado Teresa, Yeo Robert P, Melero José A
Abstract excerpt
The reactivity of a panel of 12 monoclonal antibodies raised against the human respiratory syncytial virus 22 kDa (22K) protein was tested by Western blotting with a set of 22K deletion mutants. The results obtained identified sequences in the C-terminal half of the 22K polypeptide required for integrity of most antibody epitopes, except for epitope 112, which was lost in mutants with short N-terminal deletions....
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