Article
Purification and in vitro selection of rosette-positive (R+) and rosette-negative (R-) phenotypes of knob-positive Plasmodium falciparum parasites.
The American journal of tropical medicine and hygiene - 1 Apr 1992
Handunnetti S M, Gilladoga A D, van Schravendijk M R, Nakamura K, Aikawa M, Howard R J
Abstract excerpt
We have developed methods for in vitro selection of Plasmodium falciparum parasites that bear knob protrusions (K+) and are either of the rosette-positive (K+R+) or rosette-negative (K+R-) phenotypes. Cryopreserved parasites from spleen-intact Aotus monkeys that were K+, C32 cell adherence-positi...
Topics
- Animals
- Aotus trivirgatus
- Cell Adhesion
- Cells, Cultured
- Centrifugation, Density Gradient
- Erythrocytes
- Humans
- Microscopy, Electron
- Phenotype
- Plasmodium falciparum
- Rosette Formation