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Detection of Human Picornaviruses by Multiplex Reverse Transcription-PCR and Liquid Hybridization

1 Mar 2005

Abstract excerpt

A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all human picornaviruses were covered. The amplicons, which represented the 5' untranslated regions...

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PMID: 15750090
DOI: 10.1128/jcm.43.3.1239-1245.2005

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