Article
Development of a reverse transcription-quantitative PCR system for detection and genotyping of aichi viruses in clinical and environmental samples.
Applied and environmental microbiology - 1 Jul 2013
Kitajima Masaaki, Hata Akihiko, Yamashita Teruo, Haramoto Eiji, Minagawa Hiroko, Katayama Hiroyuki
Abstract excerpt
Aichi viruses (AiVs) have been proposed as a causative agent of human gastroenteritis potentially transmitted by fecal-oral routes through contaminated food or water. In the present study, we developed a TaqMan minor groove binder (MGB)-based reverse transcription-quantitative PCR (RT-qPCR) system that is able to quantify AiVs and differentiate between genotypes A and B. This system consists of two assays, an AiV...
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