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Article

A novel enterovirus and parechovirus multiplex one-step real-time PCR-validation and clinical experience.

Journal of virological methods - 1 Nov 2013

Nielsen Alex Christian Yde, Böttiger Blenda, Midgley Sofie Elisabeth, Nielsen Lars Peter

Abstract excerpt

As the number of new enteroviruses and human parechoviruses seems ever growing, the necessity for updated diagnostics is relevant. We have updated an enterovirus assay and combined it with a previously published assay for human parechovirus resulting in a multiplex one-step RT-PCR assay. The multiplex assay was validated by analysing the sensitivity and specificity of the assay compared to the respective monoplex...

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Identifiers

PMID: 23845901
DOI: 10.1016/j.jviromet.2013.06.038
PMCID: PMC7119552

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