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Article

Evaluation of opa-Based Real-Time PCR for Detection of Neisseria gonorrhoeae

18 Feb 2005

Abstract excerpt

OBJECTIVES: Detection of Neisseria gonorrhoeae by commercial and in-house-based assays has been hampered by false-positive and false-negative results. The current study describes a sensitive and specific real-time 5'-nuclease PCR assay targeting a 90-bp region of the multicopy opa gene. GOAL: To evaluate the sensitivity and specificity of this assay in detection of gonococcus. STUDY: Sensitivity and specificity...

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PMID: 15729160
DOI: 10.1097/01.olq.0000154495.24519.bf

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