Article
Involvement of specific proteins (Sp1/Sp3) and nuclear factor Y in basal transcription of the distal promoter of the rat pyruvate carboxylase gene in beta-cells.
Biochemical and biophysical research communications - 1 Apr 2005
Sunyakumthorn Piyanate, Boonsaen Thirajit, Boonsaeng Vichai, Wallace John C, Jitrapakdee Sarawut
Abstract excerpt
Pyruvate carboxylase plays diverse roles in different biosynthetic pathways, including glucose-induced insulin secretion in pancreatic beta-cells. We have localized the control region of the P2 promoter by generating a series of 5'-nested deletion constructs, and both 25- and 9-bp internal deletion constructs, as well as by performing site-directed mutagenesis. Transient transfections of these constructs into...
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