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Molecular Diagnosis of Ewing??s Sarcoma/Primitive Neuroectodermal Tumor in Formalin-Fixed Paraffin-Embedded Tissues by RT-PCR and Fluorescence In Situ Hybridization

14 Feb 2005

Abstract excerpt

Recent studies have suggested that formalin-fixed paraffin-embedded (FFPE) tissues can be used for molecular analyses by fluorescence in situ hybridization (FISH) and RT-PCR. We analyzed 18 cases of ES/PNET for the t(11;22)(q24;q12) and t(21;22)(q22;q12) fusion transcripts by RT-PCR and analyzed for EWS translocation by interphase FISH with a dual color fusion probe to compare these two approaches directly....

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PMID: 15714060
DOI: 10.1097/01.pdm.0000140192.27878.97

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