Article
Experimental and simulative dissociation of dimeric Cu,Zn superoxide dismutase doubly mutated at the intersubunit surface.
Biophysical journal - 1 Apr 2005
Maragliano L, Falconi M, Sergi A, Cioni P, Castelli S, Lania A, Stroppolo M E, Strambini G, Ferrario M, Desideri A
Abstract excerpt
The equilibrium properties of dimeric Photobacterium leiognathi Cu,Zn superoxide dismutase mutant bearing two negative charges in the amino acid clusters at the association interface has been studied, experimentally and computationally, and compared to those of the native enzyme. Pressure-dependent dissociation is observed for the mutant, as observed by the fluorescence shift of the unique tryptophan residue...
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