Article
Phylogenetic internal control for HIV-1 genotypic antiretroviral testing.
The new microbiologica - 1 Apr 2004
Boeri E, Canducci F, Grasso M A, Presi S, Carrera P, Racca S, Clementi M
Abstract excerpt
Genotypic testing includes several steps (RNA purification, RT-PCR amplification, DNA sequencing, sequence editing and analysis) that should be individually controlled. In our laboratory, we have added to this step-by-step internal control a final phylogenetic quality control: this is performed every time a sequence is obtained from a patient previously subjected to the same test. Each sequence with this...
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