Article
Identification of transcripts by macroarrays, RT–PCR and in situ hybridization in human ejaculate spermatozoa
1 Feb 2005
Abstract excerpt
Round spermatids contain high levels of extremely varied mRNAs that are synthesized either throughout early spermatogenesis or during spermiogenesis from the haploid genome. Concomitantly, with major changes in the chromatin organization, arrest of transcription occurs at midspermiogenesis. However, previous investigations using RT-PCR have revealed the persistence of numerous and different transcripts in...
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