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Article

Characterization and quantification of mRNA transcripts in ejaculated spermatozoa of fertile men by serial analysis of gene expression

24 Feb 2006

Abstract excerpt

BACKGROUND: Accumulated evidence proves that mature spermatozoa contain a complex yet specific array of mRNA, which could provide information on the past events of spermatogenesis. OBJECTIVE: To quantitatively microdissect these mRNA transcripts by a digital approach. METHODS: Serial analysis of gene expression (SAGE) was used to study the mRNA transcripts from ejaculate of a fertile individual and of a pool of...

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PMID: 16501037
DOI: 10.1093/humrep/del027

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