Article
Protein engineering of cytochrome b562 for quinone binding and light-induced electron transfer.
Proceedings of the National Academy of Sciences of the United States of America - 21 Dec 2004
Hay Sam, Wallace Brett B, Smith Trevor A, Ghiggino Kenneth P, Wydrzynski Tom
Abstract excerpt
The central photochemical reaction in photosystem II of green algae and plants and the reaction center of some photosynthetic bacteria involves a one-electron transfer from a light-activated chlorin complex to a bound quinone molecule. Through protein engineering, we have been able to modify a protein to mimic this reaction. A unique quinone-binding site was engineered into the Escherichia coli cytochrome b(562)...
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