Low‐temperature pausing of cultivated mammalian cells

doi:10.1002/bit.20320

Article

Low‐temperature pausing of cultivated mammalian cells

6 Dec 2004

Abstract excerpt

There are currently two methods for maintaining cultured mammalian cells, continuous passage at 37 degrees C and freezing in small batches. We investigated a third approach, the "pausing" of cells for days or weeks at temperatures below 37 degrees C in a variety of cultivation vessels. High cell viability and exponential growth were observed after pausing a recombinant Chinese hamster ovary cell line (CHO-Clone...

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