Article
The optimization of a rapid pulsed-field gel electrophoresis protocol for the typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp.
Japanese journal of infectious diseases - 1 Sept 2009
Durmaz Riza, Otlu Baris, Koksal Fatih, Hosoglu Salih, Ozturk Recep, Ersoy Yasemin, Aktas Elif, Gursoy Nafia Canan, Caliskan Ahmet
Abstract excerpt
Pulsed-field gel electrophoresis (PFGE) is the most common genotyping method used for the typing of a number of bacterial species. Generally, investigators use their own custom-developed protocol, but a standardized PFGE protocol would allow the comparison of typing results between laboratories a...
Topics
- Acinetobacter baumannii
- Animals
- Bacterial Typing Techniques
- Cluster Analysis
- DNA Fingerprinting
- Electrophoresis, Gel, Pulsed-Field
- Escherichia coli
- Genotype
- Klebsiella
- Molecular Epidemiology
- Reproducibility of Results
- Time Factors