Article
The optimization of a rapid pulsed-field gel electrophoresis protocol for the typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp.
Japanese journal of infectious diseases - 1 Sept 2009
Durmaz Riza, Otlu Baris, Koksal Fatih, Hosoglu Salih, Ozturk Recep, Ersoy Yasemin, Aktas Elif, Gursoy Nafia Canan, Caliskan Ahmet
Abstract excerpt
Pulsed-field gel electrophoresis (PFGE) is the most common genotyping method used for the typing of a number of bacterial species. Generally, investigators use their own custom-developed protocol, but a standardized PFGE protocol would allow the comparison of typing results between laboratories and the tracing of strains around the country. In the present study, we optimized a PFGE protocol for subtyping of...
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