Article
Mining of unexplored habitats for novel chitinases--chiA as a helper gene proxy in metagenomics.
Applied microbiology and biotechnology - 1 Jun 2012
Cretoiu Mariana Silvia, Kielak Anna Maria, Abu Al-Soud Waleed, Sørensen Søren J, van Elsas Jan Dirk
Abstract excerpt
The main objective of this study was to assess the abundance and diversity of chitin-degrading microbial communities in ten terrestrial and aquatic habitats in order to provide guidance to the subsequent exploration of such environments for novel chitinolytic enzymes. A combined protocol which encompassed (1) classical overall enzymatic assays, (2) chiA gene abundance measurement by qPCR, (3) chiA gene...
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