Article
Culture-independent analysis of bacterial species from an anaerobic mat from Lake Fryxell, Antarctica: prokaryotic diversity revisited.
Cellular and molecular biology (Noisy-le-Grand, France) - 1 Jul 2004
Stackebrandt E, Brambilla E, Cousin S, Dirks W, Pukall R
Abstract excerpt
Using a lake sediment mat sample from Lake Fryxell, Antarctica, different DNA extraction and purification methods were compared by denaturing gradient gel electrophoresis (DGGE). Based on the analyses of cloned 16S rRNA gene sequences a high degree of as yet uncultured prokaryotes have been reported in this sample. Although the vast majority of these as yet uncultured organisms seem to be classified as...
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