Article
Bacterial diversity assessment in Antarctic terrestrial and aquatic microbial mats: a comparison between bidirectional pyrosequencing and cultivation.
PloS one - 1 Jan 2014
Tytgat Bjorn, Verleyen Elie, Obbels Dagmar, Peeters Karolien, De Wever Aaike, D'hondt Sofie, De Meyer Tim, Van Criekinge Wim, Vyverman Wim, Willems Anne
Abstract excerpt
The application of high-throughput sequencing of the 16S rRNA gene has increased the size of microbial diversity datasets by several orders of magnitude, providing improved access to the rare biosphere compared with cultivation-based approaches and more established cultivation-independent techniq...
Topics
- Antarctic Regions
- Bacteria
- Base Composition
- Databases, Nucleic Acid
- Genetic Variation
- Phylogeny
- RNA, Ribosomal, 16S
- Seawater
- Sequence Analysis, DNA
- Temperature