Article
Cloning and expression of Mycobacterium aurum carotenogenesis genes in Mycobacterium smegmatis.
FEMS microbiology letters - 15 Jan 1992
Houssaini-Iraqui M, Lazraq M H, Clavel-Sérès S, Rastogi N, David H L
Abstract excerpt
This report describes the first successful transfer and complete expression of clustered mycobacterial genes controlling a biosynthetic pathway (carotenogenesis) in a homologous system. A genomic library of pigmented Mycobacterium aurum A+ (yellow-orange) DNA was constructed in shuttle vector pHLD-69. The colourless mutant A11 and the brick-red mutant NgR9 derived from M. aurum A+ were electroporated with the...
Topics
- Carotenoids
- Cloning, Molecular
- Gene Expression
- Genes, Bacterial
- Genetic Vectors
- Mutation
- Mycobacterium