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Article

Rapid detection of Mycoplasma dispar and M. bovirhinis using allele specific polymerase chain reaction protocols.

FEMS microbiology letters - 1 Dec 2004

Miles Katie, McAuliffe Laura, Ayling Roger D, Nicholas Robin A J

Abstract excerpt

We describe an allele specific PCR based approach for the rapid detection of two bovine Mycoplasma species associated with respiratory disease. Specific and universal oligonucleotides were used in combination to detect the presence of single nucleotide polymorphisms within the 16S ribosomal DNA sequence. Presence of Mycoplasma 16S rDNA is indicated by the production of a single control fragment, whilst positive...

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PMID: 15556716
DOI: 10.1016/j.femsle.2004.10.010

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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