Article
Detection of Mycoplasma bovis and Mycoplasma agalactiae by oligonucleotide probes complementary to 16S rRNA.
Molecular and cellular probes - 1 Feb 1991
Mattsson J G, Gersdorf H, Göbel U B, Johansson K E
Abstract excerpt
The partial sequences of 16S rRNA from Mycoplasma bovis and M. agalactiae were determined by dideoxynucleotide sequencing using reverse transcriptase. Two oligonucleotides complementary to different evolutionary variable regions of 16S rRNA from these two species were synthesized. The oligonucleotides were end-labelled with 32P and used as probes in filter hybridization experiments with different bovine, caprine...
Topics
- Animals
- Autoradiography
- Base Sequence
- Cattle
- Genetic Variation
- Goats
- Molecular Sequence Data
- Mycoplasma
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- RNA, Bacterial