Article
Development and evaluation of a novel single-nucleotide-polymorphism real-time PCR assay for rapid detection of fluoroquinolone-resistant Mycoplasma bovis.
Journal of clinical microbiology - 1 Aug 2010
Ben Shabat M, Mikula I, Gerchman I, Lysnyansky I
Abstract excerpt
Monitoring of the susceptibility of Mycoplasma bovis field isolates to antibiotics is important for the appropriate choice of treatment. However, in vitro susceptibility testing of mycoplasmas is technically demanding and time-consuming, especially for clinical isolates, and is rarely performed in mycoplasma diagnostic laboratories. Thus, the development of methods allowing rapid real-time detection of resistant...
Topics
- Animals
- Anti-Bacterial Agents
- Bacterial Proteins
- Cattle
- Cattle Diseases
- DNA Topoisomerase IV
- DNA, Bacterial
- Fluoroquinolones
- Genotype
- Microbial Sensitivity Tests
- Mycoplasma Infections
- Mycoplasma bovis
- Oligonucleotide Probes