Article
Peripheral myelin protein 22 kDa and protein zero: domain specific trans-interactions.
Molecular and cellular neurosciences - 1 Dec 2004
Hasse Birgit, Bosse Frank, Hanenberg Helmut, Müller Hans Werner
Abstract excerpt
The peripheral myelin proteins P0 and PMP22 are associated in preparations of compact myelin and in cell cultures coexpressing both molecules. The mechanism of this interaction, however, still needs to be unravelled. We have established three different (cell-cell, cell-protein, protein-protein based) assay systems using retrovirally transduced HeLa cells that overexpressed either PMP22 or P0 and purified GST...
Topics
- Biological Assay
- Cell Adhesion
- Cell Membrane
- Charcot-Marie-Tooth Disease
- Genetic Vectors
- HeLa Cells
- Hereditary Sensory and Motor Neuropathy
- Humans
- Molecular Biology
- Mutation
- Myelin P0 Protein
- Myelin Proteins
- Myelin Sheath
- Peripheral Nerves
- Protein Binding
- Protein Structure, Tertiary
- Recombinant Fusion Proteins
- Transduction, Genetic