In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase.

doi:10.1074/jbc.M406962200

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Article

In vitro phosphorylation by cAMP-dependent protein kinase up-regulates recombinant Saccharomyces cerevisiae mannosylphosphodolichol synthase.

The Journal of biological chemistry - 11 Feb 2005

Banerjee Dipak K, Carrasquillo Elena A, Hughey Patsy, Schutzbach John S, Martínez Juan A, Baksi Krishna

Abstract excerpt

DPM1 is the structural gene for mannosylphosphodolichol synthase (i.e. Dol-P-Man synthase, DPMS) in Saccharomyces cerevisiae. Earlier studies with cDNA cloning and sequence analysis have established that 31-kDa DPMS of S. cerevisiae contains a consensus sequence (YRRVIS141) that can be phosphorylated by cAMP-dependent protein kinase (PKA). We have been studying the up-regulation of DPMS activity by protein kinase...

Topics

Catalysis
Cloning, Molecular
Cyclic AMP
Cyclic AMP-Dependent Protein Kinases
DNA, Complementary
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression Regulation, Fungal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Vectors

Guanosine Diphosphate

Immunoblotting

Kinetics

Mannose

Mannosyltransferases

Mutagenesis, Site-Directed

Mutation

Phosphorylation

Source

Open in PubMed

Identifiers

PMID: 15548536
DOI: 10.1074/jbc.M406962200

Genes

DPM1
SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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