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Structural Requirements of Astrovirus Virus-Like Particles Assembled in Insect Cells

12 Nov 2004

Abstract excerpt

Expression of the complete ORF2 of human astrovirus serotype 1 (HAstV-1) in the baculovirus system led to the formation of virus-like particles (VLPs) of around 38 nm. The same kind of VLPs were also obtained either with the expression of a truncated form of ORF2 lacking the first 70 amino acids (aa), or with the same truncated form in which those 70 aa were replaced by the green fluorescent protein. All three...

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PMID: 15542679
DOI: 10.1128/jvi.78.23.13285-13292.2004

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