Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Dissecting and designing inhibitor selectivity determinants at the S1 site using an artificial Ala190 protease (Ala190 uPA).

Journal of molecular biology - 19 Nov 2004

Katz Bradley A, Luong Christine, Ho Joseph D, Somoza John R, Gjerstad Erik, Tang Jie, Williams Steven R, Verner Erik, Mackman Richard L, Young Wendy B, Sprengeler Paul A, Chan Hedy, Mortara Kyle, Janc James W, McGrath Mary E

Abstract excerpt

A site-directed mutant of the serine protease urokinase-type plasminogen activator (uPA), was produced to assess the contribution of the Ser190 side-chain to the affinity and selectivity of lead uPA inhibitors in the absence of other differences present in comparisons of natural proteases. Crysta...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alanine
Share to Benzimidazoles

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Drug Design

Share to Guanidine

Share to Hydrogen Bonding

Share to Indoles

Share to Protease Inhibitors

Share to Urokinase-Type Plasminogen Activator

Source

Identifiers

PMID: 15522303
DOI: 10.1016/j.jmb.2004.09.032

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-15522303-2026-09-04.1. Manifest publication-graph-cohort-2026-09-04.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Dissecting and designing inhibitor selectivity determinants at the S1 site using an artificial Ala190 protease (Ala190 uPA).PMID 15522303Read ↗

Plasminogen activator inhibitor-2 is highly tolerant to P8 residue substitution--implications for serpin mechanistic model and prediction of nsSNP activities.Semantic proximity Read ↗

Understanding binding selectivity toward trypsin and factor Xa: the role of aromatic interactions.Semantic proximity Read ↗

Structural determinants of RXPA380, a potent and highly selective inhibitor of the angiotensin-converting enzyme C-domain.Semantic proximity Read ↗

Structural basis for recognition of urokinase-type plasminogen activator by plasminogen activator inhibitor-1.Semantic proximity Read ↗

Kinetic and structural characterization of mutations of glycine 216 in alpha-lytic protease: a new target for engineering substrate specificity.Semantic proximity Read ↗

The influence of angiotensin converting enzyme mutations on the kinetics and dynamics of N-domain selective inhibition.Semantic proximity Read ↗

Identification of serpin determinants of specificity and selectivity for furin inhibition through studies of α1PDX (α1-protease inhibitor Portland)-serpin B8 and furin active-site loop chimeras.Semantic proximity Read ↗

Purification and characterization of S1 mutants of alpha-lytic protease having altered catalytic properties.Semantic proximity Read ↗

Role of P225 and the C136-C201 disulfide bond in tissue plasminogen activator.Semantic proximity Read ↗

Dual-reporter high-throughput screen for small-molecule in vivo inhibitors of plasminogen activator inhibitor type-1 yields a clinical lead candidateSemantic proximity Read ↗

How a Fragment Draws Attention to Selectivity Discriminating Features between the Related Proteases Trypsin and Thrombin.Semantic proximity Read ↗

The distal hinge of the reactive site loop and its proximity: a target to modulate plasminogen activator inhibitor-1 activity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.