Article
PCR method for genotyping and zygosity-testing of RasH2 transgenic mice.
Experimental animals - 1 Oct 2004
Suemizu Hiroshi, Kito-Maruyama Chika, Sotomaru Yusuke, Ogura Tomoyuki, Hioki Kyoji, Ohnishi Yasuyuki, Tamaoki Norikazu
Abstract excerpt
In short-term carcinogenicity testing using CB6F1-TgrasH2 mice, sibling nonTgrasH2 mice are used as a negative control. However, selection of TgrasH2 and nonTgrasH2 mice has been performed by PCR with only transgene specific primers by the conventional method. Therefore, the conventional method i...
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