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Article

Real-time characterization of virulence factor expression in Yersinia pestis using a GFP reporter system.

Biochemical and biophysical research communications - 12 Nov 2004

Forde Cameron E, Rocco Jennifer M, Fitch J Patrick, McCutchen-Maloney Sandra L

Abstract excerpt

A real-time reporter system was developed to monitor the thermal induction of virulence factors in Yersinia pestis, the etiological agent of plague. The reporter system consists of a plasmid in Y. pestis in which the expression of green fluorescent protein (GFP) is under the control of the promoters for six virulence factors, yopE, sycE, yopK, yopT, yscN, and lcrE yopN, which are all components of the Type III...

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PMID: 15474497
DOI: 10.1016/j.bbrc.2004.08.236

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Real-time characterization of virulence factor expression in Yersinia pestis using a GFP reporter system.PMID 15474497Read ↗

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Virulence plasmid-encoded YopK is essential for Yersinia pseudotuberculosis to cause systemic infection in miceSemantic proximity Read ↗

YscB of Yersinia pestis functions as a specific chaperone for YopN.Semantic proximity Read ↗

Gain-of-Function Analysis Reveals Important Virulence Roles for the Yersinia pestis Type III Secretion System Effectors YopJ, YopT, and YpkA.Semantic proximity Read ↗

Modulation of virulence factor expression by pathogen target cell contact.Semantic proximity Read ↗

A novel protein, LcrQ, involved in the low-calcium response of Yersinia pseudotuberculosis shows extensive homology to YopH.Semantic proximity Read ↗

YopM inhibits platelet aggregation and is necessary for virulence of Yersinia pestis in mice.Semantic proximity Read ↗

lcrR, a low-Ca2(+)-response locus with dual Ca2(+)-dependent functions in Yersinia pestis.Semantic proximity Read ↗

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