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Detection of Bacterial Endotoxin in Human Tissues

1 Sept 2004

Abstract excerpt

Detection of bacterial lipopolysaccharide (LPS) in the absence of overt infection is a challenging problem in tissue homogenates and other complex samples. We found that conventional Limulus amebocyte lysate (LAL) assays are not suitable for this purpose due to interference from beta-glucan-like molecules. In contrast, a modified LAL assay that is unaffected by beta-glucan-like molecules was able to detect LPS in...

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PMID: 15470896
DOI: 10.2144/04373st06

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