Article
A structured mutant population for forward and reverse genetics in Barley (Hordeum vulgare L.).
The Plant journal : for cell and molecular biology - 1 Oct 2004
Caldwell David G, McCallum Nicola, Shaw Paul, Muehlbauer Gary J, Marshall David F, Waugh Robbie
Abstract excerpt
Two large-scale ethylmethanesulfonate (EMS) mutant populations from barley (Hordeum vulgare L.) cv. Optic have been developed to promote both forward and reverse genetics in this crop. Leaf material and seed from approximately 20 000 M(2) plants were individually harvested, freeze-dried and archived. DNA was isolated from 9216 plants from the 20 and 30 mm EMS treatments and assembled into 1152 eight-plant pools....
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