Article
Identification of the catalytic base in long chain acyl-CoA dehydrogenase.
Biochemistry - 12 Apr 1994
Djordjevic S, Dong Y, Paschke R, Frerman F E, Strauss A W, Kim J J
Abstract excerpt
We have used molecular modeling and site-directed mutagenesis to identify the catalytic residues of human long chain acyl-CoA dehydrogenase. Among the acyl-CoA dehydrogenases, a family of flavoenzymes involved in beta-oxidation of fatty acids, only the three-dimensional structure of the medium ch...
Topics
- Acyl-CoA Dehydrogenase, Long-Chain
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Catalysis
- Cloning, Molecular
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Rats
- Sequence Homology, Amino Acid
- Spectrophotometry, Ultraviolet