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Article

Nonsense-mediated mRNA decay in barley mutants allows the cloning of mutated genes by a microarray approach.

Plant physiology and biochemistry : PPB - 1 Jan 2000

Gadjieva Rena, Axelsson Eva, Olsson Ulf, Vallon-Christersson Johan, Hansson Mats

Abstract excerpt

We have previously described a microarray approach to identify and clone genes from mutants of higher organisms. In the method cDNA of two mutants with similar phenotype are competitively hybridized to DNA clones arrayed on a glass slide. Clones corresponding to an mRNA that is not expressed in one of the strains due to a mutation will be specifically highlighted in the hybridization, which provides a possibility...

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Identifiers

PMID: 15331098
DOI: 10.1016/j.plaphy.2004.06.005

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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