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Uncultured soil bacteria are a reservoir of new antibiotic resistance genes.

Environmental microbiology - 1 Sept 2004

Riesenfeld Christian S, Goodman Robert M, Handelsman Jo

Abstract excerpt

Antibiotic resistance genes are typically isolated by cloning from cultured bacteria or by polymerase chain reaction (PCR) amplification from environmental samples. These methods do not access the potential reservoir of undiscovered antibiotic resistance genes harboured by soil bacteria because most soil bacteria are not cultured readily, and PCR detection of antibiotic resistance genes depends on primers that...

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PMID: 15305923
DOI: 10.1111/j.1462-2920.2004.00664.x

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